Beilstein J. Org. Chem.2019,15, 551–557, doi:10.3762/bjoc.15.49
to build synthetic metabolism. Here I discuss the current challenges and limitations in synthetic metabolic engineering and elucidate how modern day enzymology can help to build a synthetic metabolism of the future.
Keywords: enzymes; invitrobiochemistry; metabolic engineering; synthetic biology
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Graphical Abstract
Figure 1:
The five levels of metabolic engineering and their definitions according to [11]. The enzyme solution s...